RBS

Part:BBa_J61140

Designed by: John Anderson   Group: Arkin Lab   (2007-09-04)

{rbs1} Library Member in BBb

The {rbs1-*} family of ribosome binding sites are type 1 ribosome binding sites in the BBb (BamHI/BglII) format. Each rbs is successively weaker in the series.

     Registry  Plasmid     Part         Description Sequence
     J61140    pBca1101    Bca1106-A    {rbs1-A}    GATCTGGCTAACATAGGGTG
     J61141    pBca1101    Bca1106-B    {rbs1-B}    GATCTGGCTAACTGAGGATG
     J61142    pBca1101    Bca1106-C    {rbs1-C}    GATCTGGCTAACCCAGGGTG
     J61143    pBca1101    Bca1106-D    {rbs1-D}    GATCTGGCTAACTCAGGTGG
     J61144    pBca1101    Bca1106-E    {rbs1-E}    GATCTGGCTAACCCTGGTAG
     J61145    pBca1101    Bca1106-F    {rbs1-F}    GATCTGGCTAACTTGGGACG
     J61146    pBca1101    Bca1106-G    {rbs1-G}    GATCTGGCTAACGCAGGTCG
     J61147    pBca1101    Bca1106-H    {rbs1-H}    GATCTGGCTAACATCGGTGG

Template:BBbRBS1Family


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//rbs/prokaryote/constitutive/miscellaneous
//regulation/constitutive
//ribosome/prokaryote/ecoli
Parameters
None